Expanding the synthetic biology toolbox for environmental bacteria

Synthetic biology deals with the design, construction, and deconstruction of biological systems―generally from basic principles and components―with the aim of understanding their functioning and to engineer novel properties. The development of synthetic biology tools specifically adapted to soil bacteria is an underlining activity of all the projects in our group, and we are constantly designing, expanding, and improving the toolbox for P. putida to facilitate the construction and control of metabolic pathways. By adhering to the principles of the Standard European Vector Architecture, we are particularly interested in the development of novel tools for orthogonal control of gene expression, genome engineering, multi-target transcriptional interference, transcriptional cascading, and post-transcriptional fine-tuning of metabolic pathways.